Indian Society of Genetics & Plant Breeding

Pattern of genetic relationship as revealed by AFLP markers in Indian sorghum [Sorghum bicolor (L.) Moench]

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Genetic relationships were assessed in 24 Indian sorghum
accessions including released varieties, hybrids and their
parental lines using fluorescent based Amplified Fragment
Length Polymorphism (AFLP) technique. Nine AFLP primer
combinations resulted in a total of 1246 amplification
products. The number of polymorphic markers per primer
combination ranged from 87 (E-ACT/M-CTG) to 167 (EACT/M-CTA). The markers were scored for the presence
or absence in binary matrix and relationships among the
accessions assessed using Jaccard’s similarity
coefficient. Mean genetic similarity was estimated at 0.538
based on this set of AFLPs. The most distant two
accessions were 104 A and SPV 462 and the closest two
were CSH 16 and 27 A at Jaccard’s similarity coefficient
values of 0.261 and 0.731 respectively. The relationships,
among released varieties, hybrids and their parental lines
were in accordance with their known pedigree information.
Except 104 A, C43 and SPV 462, all the accessions were
more or less uniformly distributed because of interrelated
pedigree and common ancestors.

Keywords: Sorghum, AFLP, genetic relationships


Year: 2008
Volume: 68
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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