Indian Society of Genetics & Plant Breeding
Forty parental lines used for development of early bulking
potato varieties were analyzed for genetic divergence
using twenty RAPD primers and cytoplasmic base by using
six PCR-based markers comprising of two chloroplast
DNA deletions based (HI and H3), one plastid SSR (NTCP9)
and three mitochondrial SCAR markers. In RAPD
divergence studies there were three clear clusters, those
with andigena pedigree and JF series in pedigree formed
two separate clusters from rest of genotypes. AGB69.1
was the most distinct parent followed by the parentprogeny combination (MS/82-638 and MS/92-3128). On
plastome/chondriome analysis three andigena type
cytoplasm possessing parents (Phulwa, AGB69-1 and A98-
175) and four wild type cytoplasm possessing parents (i.e.
AG14(X37)H, J99-2, A98-165 and PS/M75) were distinct
from the rest i.e. tuberosum type cytoplasm genotypes.
The diverse andigena type cytoplasm genotypes can be
preferred as parents to broaden the genetic base in early
bulking varieties, while those wild type cytoplasm
genotypes may contribute to introgression of useful traits
like higher dry matter and late blight resistance. The results
of present study showed that divergent parents from both
paternal and maternal pedigree are being used in Indian
breeding programme for early bulking varieties. Divergent
parents identified in present study may be useful in
planning hybridization programme aimed at effective
utilization of heterosis.
Keywords: Nuclear, cytoplasmic, diversity, RAPD, potato, organelle genome
Year: 2008
Volume: 68
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906
V. P. Chimote info_circle
Rajkumar info_circle
Neha Sharma and Sonali Kamal info_circle
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