Indian Society of Genetics & Plant Breeding

New frontiers in chromosome elimination-mediated doubled haploidy breeding: Focus on speed breeding in bread and durum wheat

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Chromosome elimination – a dynamic process occurring
during wide hybridization in wheat when crossed with
various Gramineae genera, has opened new horizon in
accelerating the genetic upgradation endeavours in wheat
with enhanced precision and efficiency. Since the invention
of Hordeum bulbosum approach, some other potential
systems leading to the chromosome elimination viz., wheat
x Zea mays and wheat x Imperata cylindrica have further
enhanced the opportunities to increase the doubled haploid
production efficiency in wheat. Imperata cylindrica -
mediated chromosome elimination approach- an innovation
of this Lab has recorded a striking success not only in
bread wheat, but also succeeded in inducing haploids
appreciably in wheat x rye and triticale x wheat derivatives
as well as in the durum wheat. GISH- a novel tool of the
molecular cytogenetic approach was used to identify, detect
and track the elimination of the I. cylindrica chromosomes
in wheat x I. cylindrica hybrids at different stages of the
mitotic division which enunciated quick elimination of the
chromosomes in the first division and attributed to higher
recovery of the haploid embryos. The out come of the
improved technique has been the release of a doubled
haploid wheat variety Him Pratham. The innovative
protocols developed can enhance the DH production
efficiency in wheat and accelerate improvement.

Keywords: Chromosome elimination, doubled haploidy, Imperata cylindrica, bread wheat durum wheat


Year: 2019
Volume: 79
Issue: 1, Supplement
Article DOI: 10.31742/IJGPB.79S.1.16
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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