Indian Society of Genetics & Plant Breeding

Mutagen treatment affects the regeneration efficiency of the shoottip explant in Celosia cristata L. (Cockscomb)

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The shoot-tip explant harvested from ethyl
methanesulphonate (EMS) and gamma ray (GR)
mutagenized seedling was cultured over MS medium
fortified with NAA and BAP for five generations to amplify
the mutated sector. Mutagens reduced the regeneration
efficiency of the explant and affected its plant growth
regulator-dependence for multiple shoot induction. While
the 12d-old shoot-tip from GR-treated seedling induced
shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated
seedling induced shoots with 8.8µM BAP. The present
study establishes that the mutagens affect the regeneration
process in the explant.

Keywords: EMS, gamma ray, in vitro mutagenesis, plant growth regulator


Year: 2020
Volume: 80
Issue: 3
Article DOI: 10.31742/IJGPB.80.3.18
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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