Morphological, anatomical and molecular characterization of full-sib pseudo-F2 (F1) progenies in mulberry with resistance to bacterial leaf spot (Xanthomonas campestris pv. mori)

Published on Aug 01, 2018 02:52 PM

Bacterial leaf spot (BLS), caused by Xanthomonas
campestris pv. mori is a devastating foliar disease of
mulberry. Full-sib progenies descendent from control
cross between BLS resistant source ‘Morus rotundiloba’
and susceptible commercial cultivar ‘S-1’ were evaluated
for BLS disease reaction. The continuous population
distribution of BLS disease severity (DSI) across the
progenies indicated additive nature of the resistant trait.
Besides, morphological and anatomical characterization
of the selected resistant and susceptible progenies
showed high significant positive correlation between DSI
and stomata frequency. Significant negative correlation
of DSI was observed with leaf thickness, thickness of
upper epidermis and upper cuticle layers. RAPD profiling
with Genei primer 17 and 18 generated two unique DNA
fragments of 500bp and 450bp for five each of resistance
and susceptible progenies, respectively. The dendrogram
using the binary statistic of RAPD analysis exhibited
distant relationship between the parents as well as
resistant and susceptible progeny groups.

Keywords: Disease resistance; morpho-anatomical features; mulberry; RAPD; Xanthomonas campestris pv. mor i

Info

Year: 2011
Volume: 71
Issue: 4
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

Rita Banerjee, S. Ghosh, S. G. Doss, A. K. Saha, A. K. Bajpai and R. K. Khatri

Download PDF