Indian Society of Genetics & Plant Breeding

Monomorphic molecular markers are as informative as polymorphic molecular markers

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Molecular markers have been assisting breeders in crop
improvement. Judicious integration of molecular markers
with conventional breeding practices has contributed
numerous improved varieties. In the process of MAS,
monomorphic markers are most often eliminated from
further investigations assuming that they are noninformative. In this study, efforts were made to elucidate
information from monomorphic markers. Twenty rice
genotypes contrasting for grain zinc content were
selected. Specific markers were designed to the already
reported genes associated with micronutrient transport
from soil to grain. Among designed markers, only OsZIP6c
and OsZIP3b were polymorphic and Single-Marker
Analysis revealed that OsZIP6c was associated with culm
zinc content. Sequencing of amplicons of six markers
including four monomorphic markers revealed that
nucleotide variations were present in monomorphic
markers as well. SMA for nucleotide variations revealed
that OsZIP5b, a monomorphic marker on agarose,
contained nucleotide changes significantly associated
with grain zinc content. This clearly demonstrates that
monomorphic markers are as informative as polymorphic
markers and should not be eliminated from the study.

Keywords: Bioinformatics, MAS, molecular markers, monomorphism, polymorphism, rice, sequencing, SMA, zinc

Info

Year: 2014
Volume: 74
Issue: Supplementry Issue
 Article DOI: 10.5958/0975-6906.2014.00896.7
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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