Molecular tagging of photoperiod responsive flowering in Indian bean [Lablab purpureus (L.) Sweet]

Published on Jun 20, 2019 11:41 AM

Photoperiod responsive flowering and growth habit might
have played a key role in domestication of lablab bean
(Lablab purpureus) and currently shifting its cultivation
from intercropping to monoculture. Most of the landraces
of lablab bean exhibit photoperiod sensitivity in flowering
and indeterminate growth habit. A cross was made between
GNIB21 and GP189 which are phenotypic extremes for
photoperiod responsive flowering. The F1 hybrid exhibited
dominant traits like indeterminate growth habit and photosensitive flowering endowed from male parent. Segregation
pattern of 3:1 in F2 generation indicated monogenic
recessive nature of photoperiod insensitive flowering. Bulk
segregant analysis in F2 population revealed association
of PvTFLy1, a locus governing determinate growth habit in
lablab bean, with photoperiod responsive flowering where
an amplicon of 300 bp was observed in photo sensitive
GP189 while it was absent in photo insensitive variety
GNIB21. Significant ÷2 test indicated coupling phase of
linkage between PvTFLY1 and photoperiod responsive
flowering. Linkage analysis placed PvTFLY1 at the distance
of 19.23 cM from the locus governing photoperiod
responsive flowering. The linkage between growth habit
and photoperiod responsive flowering in common bean,
soybean and Indian bean suggest that these traits may be
governed by mutation or deletion of E3 (or GmPhyA3) and
Dt1 homologs in Indian bean. Information available on
characterized genes for photoperiod responsive flowering
and determinate growth habit from common bean, soybean
and other related legumes may be utilized for isolation,
characterization, mapping and molecular dissection of
genes involved in regulation of photoperiod responsive
flowering in Indian bean.

Keywords: Molecular tagging, photoperiod responsive flowering, bulk segregant analysis, terminal flowering locus, linkage analysis

Info

Year: 2019
Volume: 79
Issue: 1, Supplement
Article DOI: 10.31742/IJGPB.79S.1.17
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

Vinita Ramtekey*, Arpit Bhuriya, Dipendra Ayer, Vipulkumar Parekh, Kaushal Modha, Bhushan Kale, Gopal Vadodariya and Ritesh Patel

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