Lutein is one of the major carotenoids in eye macula and
its deficiency is attributed to age-related macular
degeneration (AMD) and cataracts. Developing Lutein rich
staple food crop will help in supplementing its requirement
among rural people through regular diet. The present study
was undertaken with a view to tag genetic loci controlling
lutein accumulation in rice through Bulked Segregant
Analysis (BSA). Estimation of lutein content in the
dehusked grains of selected 65 RILs revealed the normal
distribution (1.14-285.62 μg/100gm) of lutein accumulation
with a significance of p<0.041. Parental polymorphism
survey using > 350 genome wide SSR markers detected
30.8% (108 SSR markers) polymorphism between Kavuni
and CO 50 rice genotypes. BSA of extreme bulks containing
contrasting levels of lutein along with the parents using
the genome wide polymorphic SSR markers resulted in the
identification of four SSR markers namely RM197 (3.0 Mb),
RM204 (3.1 Mb), RM225 (3.4 Mb) and RM19442 (3.7Mb) on
chromosome 6 showing clear association with the lutein
content. Single-marker linear regression approach using
the allelic pattern of all four markers in the region 3.0-3.7Mb
showed significant association with lutein content. The
regression analysis showed that the SSR markers in the
region 3.0-3.7Mb linked QTL accounted for 35.9% of the
genetic variation for lutein content.
Keywords: Kavuni, lutein, carotenoid, BSA, QTL
Year: 2020
Volume: 80
Issue: 1
Article DOI: 10.31742/IJGPB.80.1.2
Print ISSN: 0019-5200
Online ISSN: 0975-6906
M. Raveendran and R. Valarmathi info_circle