Indian Society of Genetics & Plant Breeding

Molecular profiling and genetic relationship among ber (Ziziphus sp.) genotypes using RAPD markers

Published on

Genetic relationship study was performed with RAPO
markers among 50
ber genotypes representing Z.
mauritiana, Z. nummularia and Z. spina-christi. Out of 120
primers initially tested, 46 were highly reproducible and
generated 368 RAPO markers with 86.2% polymorphism
(316 polymorphic bands). The number of amplification
product per primer ranged from 2 (OPF-9) to 17 (OPO-3)
with an average of 8 bands per primer. The resolving
power (Rp) for different primers ranged from 0.48 (OPE-
4) to 9.37 (OPO-3) and polymorphic information content
(PIC) ranged from 0.12 (OPO-20) to 0.82 (OPE-9). Nineteen
primers distinguished at least one genotype that would
prove to be highly useful for identification of genotype
and designing future breeding strategy. Genetic
relationships between the accessions were established
based on Jacquard's similarity coefficient and it ranged
from 26.3% to 78.9% suggesting that the
germplasm is genetically diverse. UPGMA cluster analysis
generated dendrogram with six clusters separating two
wild genotypes, Z.
nummularia (collection 1) and Z.
spina-christi from rest of the genotypes. Cluster-I and II
comprised of two genotypes each, whereas, the biggest
cluster, cluster VI comprised of 20 out of remaining 44
genotypes. In cluster II to cluster-VI, genotypes were
separated from each other at different similarity levels in
successive branching. The present study has proved that
ber genotype earlier reported to be similar based on
morphology are genetically different. The degree of genetic
variation detected in
Ziziphus species with RAPO analysis
in the present study suggests that it is an efficient marker
technology for delineating genetic relationships among
genotypes and estimating genetic diversity, thereby
enabling the formulation of appropriate strategy for
conservation and improvement programmes.

Keywords: Ziziphus sp., genetic diversity, PIC, resolving power


Year: 2007
Volume: 67
Issue: 2
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



Download PDF