Indian Society of Genetics & Plant Breeding

Molecular phylogeny of the Chinese weedy rice based on nrDNA ITS and two cpDNA sequences

Published on

Direct sequencing data from the nuclear ribosomal DNA
internal transcribed spacer (nrDNA ITS) region and two
chloroplast DNA (cpDNA) fragments (psbA-trnH and petGtrnP) of Chinese weedy rice (Oryza stavia L. f. sp. spontanea)
were analyzed separately and simultaneously in the MEGA
6.0 software. The nrDNA, cpDNA and cpDNA+nrDNA
phylogenetic trees showed that the weedy rice collected
from Northern China, Oryza sativa Japonica group and Oryza
rufipogon, were on the one phylogenic node whereas
weedy rice were collected from Southern China and Oryza
sativa Indica group were on the other phylogenic node.
The results revealed that there were geographical and
indica-japonica differentiations in Chinese weedy rice which
were corresponding to their geographical distribution and
cultivated rice (Oryza sativa) indica-japonica differentiation.
These results demonstrated that Chinese weedy rice from
different regions have diverse origins and they were the
relative origin group of the cultivated rice as there is no
wild form available in Northern China. Additionally, clone
sequence variation of the nrDNA ITS regions in intraindividual weedy rice provides evidence origin of other
Oryza taxa through the process of hybridization or
introgression. Data from this research further confirmed
that the Chinese weedy rice was evolved from local rice
varieties (as the female parent) which naturally hybridized
with other oryza materials.

Keywords: Weedy rice, nrDNA ITS region, psbA-trnH and petG-trnP intergenic spacer, molecular phylogeny, hybridization


Year: 2016
Volume: 76
Issue: 1
Article DOI: 10.5958/0975-6906.2016.00008.0
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Zhang Shiqi info_circle
Zhai Dong info_circle
Yu Fang info_circle
Jin Zhaoxia info_circle
Chen Wenfu info_circle

Download PDF