Indian Society of Genetics & Plant Breeding

Molecular marker analysis for root length in a diverse germplasm of rice (Oryza sativa L.)

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Rice (Oryza sativa L.) is among the most important crops
in the world. The productivity of rice in rainfed regions
is constrained by drought. Among the root traits
contributing to drought resistance, root system parameters
play an important role in the regulation of plant growth
through water and nutrient uptake from deep soil layers.
The present study was conducted to validate molecular
markers known to be associated with maximum root
length. Seventy-nine diverse lines and six checks were
used. Variation was observed for several root
morphological traits viz., maximum root length, total root
number, root volume, root dry weight, shoot dry weight,
plant height, number of tillers, total dry weight and root
to shoot weight ratio. Most of these traits showed
significant correlation and appeared interrelated. RAPD
and microsatellite primers, which have been established
as markers for maximum root length, were used for
screening rice genotypes. Single-marker analysis showed
significant association between the markers and maximum
root length. Thus, these markers can be used for
marker-assisted selection in plant breeding programmes
involving diverse parents.

Keywords: Rice, core and donor lines, microsatellite, RAPD, root morphology


Year: 2003
Volume: 63
Issue: 3
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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