Indian Society of Genetics & Plant Breeding

Molecular diversity analysis and DNA fingerprinting of cotton varieties of India

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Genetic diversity was assessed in 48 popular varieties of
tetraploid cotton from each cultivated zone of India using
68 SSR markers distributed across linkage groups. The
markers produced a total of 144 alleles with an average of
2.19 per locus. The polymorphism information content (PIC)
ranged from 0.04 to 0.57 with a mean of 0.23 indicating
lesser diversity in the studied material. Jaccard’s similarity
index based neighbourhood joining cluster analysis
grouped the genotypes into three major clusters, each of
which was further classified into sub-groups.
Inconsistencies were observed between the clusters and
known pedigree of the cultivars. A narrow genetic base
was also revealed among the cotton cultivars. The SSR
markers revealed a genetic similarity of 73% among the
varieties studied. The DNA fingerprint developed using a
selected set of 14 markers showed a probability of identical
match of 2.47×10
-3 with high goodness of fit (r2=0.86). The
identified markers have great potential in DNA fingerprinting
in cotton which in future could be integrated with DUS
data descriptors for effective cultivar identification and
differentiation.
  

Keywords: Cotton, genetic diversity, fingerprinting, SSR markers, varieties

Info

Year: 2019
Volume: 79
Issue: 4
Article DOI: 10.31742/IJGPB.79.4.10
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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