Indian Society of Genetics & Plant Breeding
Random Amplified Polymorphic DNA (RAPD) was employed
to characterize the interspecific hybrid of G. hirsutum 76
IH x G. anomalum and test its hybrid status. Out of 24
random primers screened, 12 were selected which
generated 182 amplification products of which 98
amplicons were polymorphic. Based on the presence or
absence of DNA bands in the female parent, offspring
and male parent, the molecular markers could be classified
into seven Groups. Group I markers shared bands in both
parents and offspring, Group II markers shared bands in
female and offspring, Group III markers shared bands in
both parents, Group IV markers shared bands in male
parent and offspring; Group VII markers were present
only in male parent. Of these, only Group IV markers
(because of the presence of true distinct bands present
in male parent) are suitable for identifying the hybrid
status of the offspring. In this study, 63.3% of the RAPD
markers revealed additivity among parents and offspring
G. hirsutum 76 IH x G. anomalum, 34.7% of parental
markers (Group III, V and VII) were absent in the offspring
while 2% unique markers (Group VI) were present. The
hybrid in the present study showed Group IV markers
common with male parents, which clearly shows that DNA
based technology such as RAPD could be used with
greater speed to detect true interspecific hybrids of
Gossypium. Further the trueness of the hybrid was also
confirmed on the basis of cytomorphological observations.
The significance of this hybrid in introgression of desirable
genes from the wild species G. anomalum to cultivated
G. hirsutum is discussed
Keywords: Cotton, interspecific hybridization, RAPD
Year: 2005
Volume: 65
Issue: 3
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
S. S. Mehetre, Susan Eapen, Michille Gomez and A. R. Aher info_circle
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