Indian Society of Genetics & Plant Breeding

Microsatellite DNA marker aided diversity analysis in cowpea [Vigna unguiculata (L.) Walp]

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Eighty three cowpea genotypes were analyzed with 15
micro satellite markers to test the genetic variation. Among
the total 15 SSR primers used, 12 primers were
polymorphic and 3 primers were monomorphic. A total of
15 markers Viere obtained, among which 80% were
polymorphic and 20% were monomorphic. Genotype
specific markers were identified for some genotypes. The
clusters constructed based on SSR marker data revealed
significant genetic variation among the genotypes. The
marker detected significant polymorphism among the local
landraces as compared to the cultivated varieties.
Genotypes with resistance to rust disease and nutritionally
superior ones grouped together in separate clusters. The
results using SSR markers indicated that micro satellites
successfully unraveled the genetic variation existing in
selected cowpea genotypes.

Keywords: Cowpea, microsatellite, polymor~h~m, dendrogram, germplasm, breeding


Year: 2009
Volume: 69
Issue: 1
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Shailaja Hittalamani,B. C. Keshava Murthy info_circle
K. P. Viswanatha info_circle

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