Indian Society of Genetics & Plant Breeding

Microsatellite based genotyping and assessment of genetic divergence in upland cotton (Gossypium hirsutum)

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Nineteen upland cotton genotypes were evaluated for
genetic divergence for development of desirable hybrids.
Based on twelve phenotypic markers D
2 analysis identified
five clusters containing one to ten genotypes. Prinipal
Component Aanalysis reduced the twelve variables into
four principal components explaining 82.2% variability that
clearly indicated the genotypes, H1236, H1472, CSH 3075,
ISR 12, H1465 and H1117 were high yielder and H 1236, H
1472, H 1156 and H1476 were having better seed indices
traits. Out of 49 SSR primers, 42 were polymorphic and
amplified 173 alleles with PIC value ranged from 0 to 0.948.
The NTSYS-pc UPGMA analysis clustered into seven distinct
groups.

Keywords: Genetic diversity, D2 analysis and SSR primers

Info

Year: 2021
Volume: 81
Issue: 2
Article DOI: 10.31742/IJGPB.81.2.16
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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