Indian Society of Genetics & Plant Breeding

Microsatellite based association studies for grain mineral content in local winter (Sali) rice of Assam

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In present investigation, grains of one hundred indica rice
genotypes were analyzed for nine mineral contents namely,
phosphorus (P), potassium (K), sodium (Na), magnesium
(Mg), calcium (Ca), zinc (Zn), iron (Fe), manganese (Mn)
and copper (Cu) and genotyped using 112 SSR markers.
STRUCTURE analysis identified k=4, indicating at least four
distinct groups in a set of 100 rice accessions. Genomewide
scanning detected a total of 10 significant markertrait
associations (P<0.01), with the R2 values ranging from
8.85% to 24.25%. Three markers, RM231 on chromosome
(Chr.) 3, RM234 (chr. 7) and RM162 (chr. 6), were found to
be linked for Ca. Three SSR markers, RM3 (chr. 6), RM400
(chr. 6) and RM286 (chr. 11) were found to be associated
with Cu content. RM 311 on chr. 10 was found to be
associated with Fe content. P was found to be associated
with three SSR markers, namely, RM536, RM234 and RM600
on chromosomes 11, 7 and 1, respectively. Some novel
QTLs were also detected and implications of these results
are discussed.

Keywords: Winter rice, minerals, association mapping, SSR markers


Year: 2016
Volume: 76
Issue: 3
Article DOI: 10.5958/0975-6906.2016.00053.5
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Sunayana Rathi info_circle
H. Verma info_circle
S. Baishya info_circle
R. N. Sarma info_circle

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