Indian Society of Genetics & Plant Breeding

Marker assisted stacking of drought tolerance QTL and rust resistance genes in an elite Indian wheat genotype

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Backcross population derived from a cross between
Triticum aestivum advance breeding line PBW703
(PBW343+Lr24+Lr28+Yr10+Yr15) and Babax, a drought
tolerant line from CIMMYT was used for introgression of
drought tolerance QTL (quantitative trait loci) regions in a
leaf and stripe rust resistant background. Out of 24
polymorphic SSR markers reported to be linked to drought
tolerance in earlier studies and 15 markers showed
introgression on different chromosomes with minimum of
two and maximum of nine introgressed regions in different
BC2F1 plants. For phenotypic analysis, 364 BC2F4 progenies
from best performing selected BC2F1 plants were evaluated
for rust reaction, chlorophyll content, canopy temperature,
stay green, plant height, number of tillers per meter, number
of spikelets per spike, number of grains per spike, 100 grain
weight under restricted irrigated conditions. Correlation
analysis showed that there was significant and positive
correlation between these morphological traits.
Transgressive segregants were observed for all the traits
which were out performing the recipient parent PBW703. A
total of 42 plants carrying the Babax specific alleles for
drought tolerance QTL and all the four rust resistance genes
Lr24, Lr28, Yr10 and Yr15 were finally selected for agronomic
evaluation. Selected improved lines for drought tolerance
are being evalauted in the wheat breeding programme.

Keywords: Triticum aestivum, Babax, drought tolerance, rust resistance genes, introgressed regions, yield related traits


Year: 2018
Volume: 78
Issue: 1
Article DOI: 10.5958/0975-6906.2018.00001.9
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Sumandeep Kaur Bazzer info_circle
Satinder Kaur info_circle
Virinder Singh Sohu info_circle
K. V. Prabhu info_circle
Parveen Chhuneja info_circle

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