Indian Society of Genetics & Plant Breeding

Important

Marker assisted pyramiding of major blast resistance genes Pi9 and Pita in the genetic background of an elite Basmati rice variety, Pusa Basmati 1

Published on

Basmati is a premium quality rice of India which is highly
priced in the international market. Pusa Basmati 1, an elite
Basmati rice variety is highly susceptible to rice blast caused
by Magnaporthe oryzae. Therefore, pyramiding blast
resistance genes is essential to effectively combat the blast
disease and increase the durability of resistance genes.
The blast resistance genes Pi9 and Pita have been earlier
demonstrated to be effective in Basmati growing regions
of the country. Therefore, in the present study, monogenicnear
isogenic lines Pusa 1637-18-7-6-20 and Pusa 1633-3-8-
8-16-1 carrying Pi9 and Pita, respectively, were intercrossed
to generate pyramided lines through marker assisted
foreground, background and phenotypic selection for
recurrent parent phenotype. The pyramided lines carrying
Pi9+Pita were found to be either at par or superior to the
recurrent parent Pusa Basmati 1 for agro-morphological,
grain and cooking quality traits. Further, these pyramided
lines were also found to show resistance against three
virulent pathotypes of M. oryzae namely, Mo-nwi-kash 1,
Mo-nwi-lon2 and Mo-ei-ran1, when evaluated under artificial
inoculation conditions as well as in the natural epiphytotic
conditions of uniform blast nursery at two locations. The
developed pyramided lines are the potential sources of
blast resistance genes in the Basmati improvement program
and can also be released for commercial cultivation after
required testing.

Keywords: Basmati rice, blast resistance, gene pyramiding, marker assisted selection

Info

Year: 2015
Volume: 75
Issue: 4
Article DOI: 10.5958/0975-6906.2015.00068.1
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

info_circle
Vinay Sharma info_circle
Asif B. Shikari info_circle
U. D. Singh, G. Prakash info_circle
T. R. Sharma,N. K. Singh info_circle
Rajeev Rathour info_circle
Mukund Variar info_circle
S. K. Prashanthi info_circle
M. Nagarajan, K. K. Vinod info_circle
H. Rajashekhara info_circle

Download PDF