Indian Society of Genetics & Plant Breeding

Maize-induced haploid production from triticale x wheat crosses

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The system of maize induced haploidy was used to devise
a rapid technique for generating D/R substitutions from
1s of hexaploid triticale x bread wheat. Standard wheat x
maize protocol was ineffective. Two refinements which
included supplementing the 2,4-D (100ppm) with picloram
(100 pm) for use in post pollination tiller injection and
advancing the F1S of triticale x wheat to subsequent
generations via selfing and backcrossing proved effective.
Caryopsis formation frequency (CFF) to the tune of 52.30%,
11.59% embryo formation frequency (EFF) and 13.10%
plant regeneration frequency (PRF) were achieved. Using
above modified auxin treatment the response of F1s of
triticale x wheat (Chinese spring with Ph suppressor)
towards maize induced haploid induction was also
obtained with CFF of 17.54 and EFF of 4.65 percent. The
maize mediated haploid induction is proposed as a rapid
technique for the genetic enhancement of triticale and

Keywords: Triticum aestivum, triticale, doubled haploids


Year: 2008
Volume: 68
Issue: 4
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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