Indian Society of Genetics & Plant Breeding

Isozyme variability in Trifolium alexandrinum accessions

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The present investigation was carried out on 65 accessions
of berseem (Trifolium alexandrinum) to estimate the
genetic variability based on five enzymes – peroxidase,
acid phosphatase, esterase, super oxide dismutase and
glutamate oxalo acetate transaminase using starch gel
electrophoresis. A similarity matrix showed 95-100%
similarity among 64 accessions. Accession EC 329299
belonging to ‘Saidi’ ecotype showed 79% similarity with
other accessions. The accessions grouped in three major
clusters based on the cutting points in the dendrogram at
root level. However, phenetic line drawn at 98% identified
25 groups comprising of 1 to 10 accessions. Of these, 13
groups with more than one germplasm lines showed 100%
similarity. Morphologically distinct accessions (i.e.
multifoliates and red flowered) formed one cluster.
Monomorphic bands (13 out of 26) were in high number.
Exotic accessions also showed close similarity to many
indigenous accessions which indicated less genetic
variability among the germplasm lines introduced from
other countries. Polymorphism was recorded for 13 bands
(2 for SOD, 3 for GOT, 1 for ACP and 7 for Peroxidase).

Keywords: Isozyme, Trifolium alexandrinum, Egyptian clover


Year: 2008
Volume: 68
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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