Indian Society of Genetics & Plant Breeding
Dry and healthy seeds of a macrosperma cv., LH90-54
were treated with three doses each of ethyl methane
sulphonate (0.2, 0.4 and 0.8%) and sodium azide (0.02,
0.04 and 0.08%). EMS induced higher frequency of
macromutations, Le. 40.0 and 10.2% against 23.91 and
3.63% mutated progenies and plants of SA, respectively.
Majority of the progenies (45.24%) segregated for mutation
of one type. Among macromutations, the frequency of
chlorophyll mutations obtained was higher (61.9%) than
the morphological mutations (38.1 %). Four kinds of
chlorophyll (albina, chlorina, xantha and viridis) and twelve
kinds of morphological mutations included changes for
growth habit (compact, bushy, prostrate), foliage (narrow,
broad, rogue, tendrillar), plant height (tall, dwarf) and
maturity and flowering behaviour (early, late, sterile). EMS
induced albina, chlorina and xantha, whereas SA induced
viridis with higher frequency. The mutations for growth
habit (56.2%) and foliage (59.1 %) were induced with higher
frequency by EMS, whereas those for plant height (61.5%)
and maturity and flowering behavior (51.1%) were induced
with higher frequency by SA. On the basis of sterility,
SA was found 4.24 and 4.28 times more effective and
efficient, respectively, than EMS
Keywords: Lentil, induced mutagenesis, chemical mutagens, macromutations, mutagenic effectiveness, mutagenic efficiency
Year: 2005
Volume: 65
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
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