Indian Society of Genetics & Plant Breeding

Isolation and identification of a salt-inducible gene encoding a putative NAC-binding transcription factor from Poa pratensis

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NAC proteins play essential role in regulating various
metabolic processes including molecular signal
transduction in plants. A new NAC-binding protein gene
PpNAC encoding an NAC-type transcription factor was
isolated by RACE-PCR from Poa pratensis seedlings. Its
ORF cDNA was 837bp long, from which a protein with 278
amino acid residues was deduced with a predicted
molecular mass of 31.43kDa and a pI of 5.30. Clustering
analysis showed that PpNAC is a nuclear protein, which
belongs to OsNAC3 subfamily of the NAC protein family.
PpNAC exhibited the highest identity to SNAC1, and the
lowest identity to NAC1. The expression of PpNAC was
promoted significantly by 4oC, 37 oC, 250mM NaCl, 100 M
ABA and 10mM SA, and inhibited by 20% PEG, 100 M MeJA
and 100 M ET. PpNAC seems to participates in regulatory
processes involved in defense responses and stress
resistance in Poa pratensis.

Keywords: NAC; Transcription factors; RACE; Quantitative RT-PCR


Year: 2013
Volume: 73
Issue: 4
Article DOI: 10.5958/j.0975-6906.73.4.064
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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