Indian Society of Genetics & Plant Breeding

Isolation and characterization of drought responsive EcDehydrin7 gene from finger millet (Eleusine coracana (L.) Gaertn.)

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Finger millet is known for its drought tolerance ability.The
present investigation was carried out to explore drought
responsive genes from finger millet. To generate more
genetic and genomic information, a highly normalized cDNA
library was prepared from 12 days old stress imposed
seedlings and utilized it for identification of drought
responsive genes using cDNA macroarray. Differentially
expressing six genes (Dehydrin7, Hypothetical protein1,
Chloroplast envelope membrane protein, Hypothetical
protein2, S-adenosyl methionine decarboxylase2 and
unknown gene) were selected for qRT-PCR analysis under
different stress conditions. Gene EcDehydrin7 (Accession
No. KM096446) showed high expression under drought and
heat stress. Functional validation of this gene can provide
more information about drought tolerance mechanism in
crop plants.

Keywords: Finger millet, cDNA, EcDehydrin7, drought and heat stress


Year: 2014
Volume: 74
Issue: 4
Article DOI: 10.5958/0975-6906.2014.00870.0
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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