Inter and intra population diversity analysis in toria (Brassica rapa L.) using SSR marker

Published on May 02, 2020 08:09 AM

Six popular toria (Brassica rapa L.) lines of Assam were

used to determine the intra and inter populations diversity

using SSR markers. Application of 39, 28 loci SSR loci

revealed a total of 80 alleles. The SSR marker BRMS-029

had the highest PIC value in each population. Genetic

diversity within individuals was high and accounted for

86% of the total variation, while among population variation

was accounting for 13%. So, intra population diversity was

found higher than the inter population diversity. The

population M27 showed the highest number of alleles

(2.128), highest number of effective alleles (1.592), Shanon

information index (0.463), expected heterozygosity (0.275)

and unbiased expected heterozygosity (0.282) indicating

the presence of more heterozygotes in the population. The

dendogram showed three distinct populations (TS 36, TS

38 and Jeuti) among the six populations and other three

populations are slightly overlapping indicating gene flow.

The high genetic diversity within cultivars of B. rapa is

required to avoid inbreeding and to enable high yield. This

study provided a new insight into the exploitation of the

genetically diverse toria lines as potential resources for

future breeding programs.

Keywords: Brassica rapa, genetic diversity, SSR marker,

Year: 2020
Volume: 80
Issue: 1
Article DOI: 10.31742/IJGPB.80.1.14
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Aradhana Phukan, P. K. Barua, R. N. Sarma and N. Borah