Inheritance of fertility restoration of A4 cytoplasm in pearl millet [Pennisetum glaucum (L.) R. Br.]

Published on May 02, 2020 07:54 AM

Present investigation was carried out to study the mode of

inheritance of fertility restoration for A4 cytoplasm using

pollen fertility and seed set per cent as criterion in

determining the fertile and sterile plants. Two CMS lines of

A4 cytoplasm were crossed with two fertility restorers

generating four F1 crosses, namely, ICMA 99111 x PPMI

1003, ICMA 99111 x PPMI 1087, ICMA 03999 x PPMI 1003 and

ICMA 03999 x PPMI 1087, their F2s and backcross

generations. All the F1s were completely fertile indicating

complete fertility restoration. F2s and backcross generations

were evaluated at IARI, New Delhi and IARI Regional Centre,

Dharwad during summer 2017 and ï£2 test was applied to

test the significance. At both the locations, all the F2

segregating populations fit well into a Mendelian ratio of

15:1 indicating digenic duplicate dominance of fertility

restoring genes with ï£2 value of 0.82, 2.90, 0.04, 3.97, 4.86,

4.98, 0.02, 1.26, 3.15, 4.98, 3.15 and 0.02. The F2 hypothesis

was verified with the observed frequency of segregating

plants fitting well into 3:1 ration with ï£2 value of 5.45, 1.93,

4.93, 0.60, 2.83, 0.44, 4.94, 2.77, 3.33, 0.13, 4.08 and 1.51. It is

further confirmation of the findings that fertility restoration

is indeed governed by two duplicate genes. Association

between pollen fertility and seed set per cent was significant

and positive.

Keywords: Inheritance, A4 cytoplasm, fertility restoration, pearl millet

Year: 2020
Volume: 80
Issue: 1
Article DOI: 10.31742/IJGPB.80.1.8
Print ISSN: 0019-5200
Online ISSN: 0975-6906

J. Jorben, S. P. Singh , C. Tara Satyavathi , S. Mukesh Sankar, Jayant S. Bhat , Kumar Durgesh and M. Mallik