Indian Society of Genetics & Plant Breeding

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Inheritance and expression of Cry2Aa gene in transgenic chickpea (Cicer arietinum L.)

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The transmission and expression of Cry2Aa gene driven
by Arabidopsis Rubisco small sub unit gene (atsA1)
promoter (AraSSU) in chickpea was previously analysed
in the T1 generation of 13 independent transgenic lines. It
is desirable that transmission of transgene(s) by maternal
and paternal parents be consistent and predictable. We
selected three representative lines, BS5A, BS6H, and BS6L
to study transgene transmission and expression up to the
T6 generation. The line BS5A has tightly linked multiple
transgene inserts, while lines BS6H and BS6L appeared
to transmit one segregating transgenic insert to their
progeny. The Cry2Aa gene was inherited up to the T6
generation in all three lines tested. In the case of line BS5A,
the level of expression of Cry2Aa protein varied within
the T1 progeny. The expression of the Cry2Aa protein was
found high in progeny of the lines, BS6H and BS6L and
the lines showed stable and predictable expression of
Cry2Aa protein up to T6 generation. Surprisingly, we have
been able to identify homozygous progeny in line BS5A,
while lines BS6H and BS6L are segregating even in the T6
generation. Loss or silencing of Cry2Aa gene under the
control of AraSSU promoter was not observed and the
transgene inherited and expressed stably in chickpea

Keywords: Transgene, Cry2Aa, chickpea, inheritance, expression

Info

Year: 2012
Volume: 72
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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Thomas J. V. Higgins info_circle
Bidyut K. Sarmah info_circle

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