Indian Society of Genetics & Plant Breeding

Induced mutagenesis in blackgram [Vigna mungo (L.) Hepper]

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A wide range of chlorophyll and viable morphological
mutations affecting almost all the parts of the plant and
seed characteristics were isolated in M
2 generation of
blackgram
[Vigna mungo (L.) Hepper] variety Type 9
treated with gamma rays doses 50 to 500 Gy. The
comparative study of frequency and spectrum of
chlorophyll and viable mutations and the mutagenic
effectiveness and efficiency of gamma rays on blackgram
included two treatment conditions: 1. Irradiation of dry
seeds with 50 to 500 Gy with an interval of 50 Gy, 2.
Soaking of seeds in water for 6 hours before irradiation
with 50 to 500 Gy again with the same interval of 50 Gy.
Mutation rate indicated that 400 Gy dry treatment was
most potent in inducing chlorophyll and viable mutations.
The frequency and spectrum of the induced chlorophyll
mutations was in the order of - Chimera, chlorina, albina
and xantha. Higher doses of gamma rays treatment 500,
450 and 400 Gy showed high lethality under both the
treatment conditions - dry and soaked. The treatment of
400 Gy gamma rays under dry condition showed highest
mutagenic efficiency in inducing mutations in blackgram.
However, lower doses of gamma rays i.e., 250 Gy under
dry condition and
150 Gy under soaked condition were
also quite effective and efficient
  

Keywords: Slackgram, induced mutations, chlorophyll and viable mutations, frequency and spectrum, mutagenic effectiveness and efficiency

Info

Year: 2007
Volume: 67
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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