Indian Society of Genetics & Plant Breeding
The spectrum and frequency of chlorophyll mutations was
assessed in M2 generation in P27, an improved cultivar
of Lsthyrus sstivus with a range of gamma rays (50, 100,
150, 200, 250, 300, 350 and 400 Gy) and ethyl methane
sulphonate [EMS 0.5% (2h & 4h), 1% (2h & 4h)] doses.
Both, gamma rays and EMS induced a wider spectrum
of chlorophyll mutations. Certain chlorophyll mutations
such as chlorina, followed by chlorotica and xantha were
found more frequently than others, indicating preferential
induction of certain type of mutations. Fairly high frequency
of chlorophyll mutants was obtained with EMS than in
gamma rays. Dose dependent increase in chlorophyll
mutation rate was observed based on plant population
and segregating progenies in M2 generation. Majority of
segregating progenies yielded only one type of chlorophyll
mutation and with an increase in the number of mutational
events frequency of segregating families reduced. Both
mutagenic effectiveness and efficiency were higher at
lower doses of the mutagen. Mutagenic effectiveness, a
measure of the frequency of mutations induced per unit
dose of mutagen, and mutagenic efficiency, proportion of
mutations in relation to undesirable effects, were higher
with EMS treatments indicating EMS as more effective
and efficient mutagen than gamma rays.
Keywords: Grasspea, chlorophyll mutations, mutagenic effectiveness, mutagenic efficiency
Year: 2001
Volume: 61
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
V. N. WAGHMARE AND R. B. MEHRA info_circle
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