Indian Society of Genetics & Plant Breeding

Incorporation of a null allele of Kunitz trypsin inhibitor through molecular backcross breeding in soybean [Glycine max (L.) Merrill.]

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Trypsin inhibitors being anti-nutritional, are a major deterrent in the utilization of soybean as a human food and animal feed. For the
genetic elimination of Kunitz trypsin inhibitor, a dysfunctional null allele (
titi) of seed-specifc KTI3 gene was transferred from (NRC 101
and NRC 102) to three rust-resistant varieties, Phule Agrani, P. Sangam, and P. Kimya. Null allele-specifc
titi-420bp and linked Satt409-
170bp markers could identify two F
2, single F3 and F4 each, eleven BC1F2, ten BC1F3 and three BC2F2 titi plants with titi genotype among
the 131 plants studied. In biochemical assay, the trypsin inhibitor activity in seeds of these plants ranged from 4.03 to 9.67 mg/g
-1. Based
on both molecular and biochemical studies, it could be concluded that these 28plants were free of Kunitz trypsin inhibitor (
kti null).

Keywords: Kunitz trypsin inhibitor, Null allele, SSR marker


Year: 2021
Volume: 81
Issue: 4
Article DOI: 10.31742/ISGPB.81.4.14
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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