Indian Society of Genetics & Plant Breeding

Incompatibility studies in sugarcane (Saccharum spp.)

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The study was undertaken during the period 2001-2004 to
investigate the behaviour of the pollen tubes in the
stigmatic tract of pistil parents utilized in intervarietal
hybridization. Among the 173 breeding material screened
for occurrence of incompatibility, 36 parents were
identified self incompatible and among them nine
incompatible parents were further studied for their cross
compatibility. Pollen germination was normal in Co 86011,
Co 91004 and Co 62198 and slightly inhibited in a cross
with Co 99002. Fertilization was accomplished within 4 hr
of pollination in crosses Co 8371 x Co 86011, Co 88028 x
Co 86011. The presence of Co 86011 and Co 91004 pollen
tubes in the ovary 8h after pollination indicated the
possibility of normal fertilization, while in cross with Co
94008, restricted pollen tube growth was observed. Pollen
tube in the clone 2000-147 did not penetrate the ovary even
10 h after pollination. Self pollen did not support the
development of pollen tubes in Co 88028, Co 91004, Co
99002, Co 62198 and Co 86011. In the crosses of Co 88028
x Co 91004, Co 88032 x Co 99002 and Co 88028 x Co 86011
pollen germination and entry of pollen tube was normal.
The study indicated that there is no hurdle at the pollen
germination stage and delayed/restricted growth of pollen
tubes indicated that the barrier operates in the stylar
region of the parent. Thus, from our study it could be
inferred that i) cross-incompatibility systems are
responsible for the failure to produce successful crosses
among Co 88028 x Co 91004, Co 88028 x Co 99002.ii) self
incompatible parents could be used as safe pistil (Co 837,
Co 8371, Co 88028, Co 88025) and pollen parents (Co 62198,
Co 86011, Co 94008, Co 91004).

Keywords: Sugarcane, incompatibility, pollen, stigma


Year: 2011
Volume: 71
Issue: 1
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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