Indian Society of Genetics & Plant Breeding

In vitro screening for regeneration in cotton (Gossypium ssp.)

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Twelve cotton genotypes representing different species
and adapted to different agroclimatic conditions have been
screened for in vitro regeneration. Diploid species G.
herbaceum and G. arboreum gave higher response for in
vitro dedifferentiation than tetraploids, G. hirsutum (4X)
and G. barbadance (4X). Hypocotyl explant showed higher
response than cotyledons for callus induction. Rooting
was observed in media containing auxins to cytokinins
in a 20:1 ratio in most genotypes. This study finally
emphasized the screening large number of genotypes for
regeneration and to study genetics of regeneration using
Coker 312, the only regenerable genotype.

Keywords: Cotton, differentiation, regeneration.


Year: 2003
Volume: 63
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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