Indian Society of Genetics & Plant Breeding
Boerhaavia diffusa L., also known as santhi, or punarnava
is an important medicinal plant, belonging to the family
Nyctaginaceae. This species is said to be distributed
throughout Malwa plateau in central India, as per ayurvedic
literature, but due to extensive commercial exploitation,
the species has become vulnerable. For callus induction,
leaf explants were cultured on Murashige and Skoog (MS)
medium supplemented with various concentrations of 2,4-
dichlorophenoxy acetic acid (2, 4-D 2.26 µM-9.04 µM) and
N6-benzyladenine (BA 1.11 µM-4.44 µM), either alone or in
combinations. Calli formed within 10-12 days of culture,
followed by shoots regeneration within 20-25 days. Direct
organogenesis was achieved from nodal explants in MS
media fortified with 2,4-D (2.26 µM-9.04 µM) along with BA
(1.11 µM-4.44 µM) within 20 days. Multiple shooting was
observed during subculture of in vitro regenerated shoots
when 2,4-D was replaced with α-naphthalene acetic acid
(NAA). Rooting was achieved in MS medium fortified with
2.85 µM IAA, within 7-10 days and also on half strength MS
medium containing 2.85 µM Indole-3-acetic acid (IAA). For
hardening, regenerated plants, with roots (3-4cm) were
initially maintained on half-strength MS liquid medium
(MS1/2) without growth regulators followed by quarter
strength MS (MS1/4) liquid medium for 10 days. For
acclimatization sterile mixture of soil, sand and manure
(2:1:1) was used. Survival rate of regenerated plants was
nearly 100%.
Keywords: Boerhaavia diffusa, vulnerable, organogenesis, plant regeneration, in vitro flowering
Year: 2019
Volume: 79
Issue: 1
Article DOI: 10.31742/IJGPB.79.1.12
Print ISSN: 0019-5200
Online ISSN: 0975-6906
Ashu Pandey, Oshin Verma and Suresh Chand info_circle
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