Indian Society of Genetics & Plant Breeding

In vitro pollen germination of some wild species of pigeonpea (Cajanus cajan) using PGM droplet technique

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In vitro pollen germination is the most widely used
technique for testing viability of pollen grains in a breeding
programme. The germination medium (PGM) for pigeonpea
species belonging to tertiary gene pool viz., Cajanus
cinereus, Rhyncosia rothii and R. aureus has been modified
from the earlier recommended medium. The improvement
was done by adding poly ethylene glycol, which supported
over 90% pollen germination and pollen tube growth of
above mentioned species. It is expected that the use of the
improved PGM will facilitate inter-specific and inter-generic
hybridization imposed by biological barriers by resorting
to pollen storage (in case of temporal isolation), in vitro
fertilization and pollen selection etc. This is the first report
of in vitro germination medium for these three tertiary gene
pool species.

Keywords: Cajanus cineres, Rhyncosia rothii, R. aureus, in vitro pollen germination


Year: 2013
Volume: 73
Issue: 2
Article DOI: 10.5958/j.0975-6906.73.2.031
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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