Indian Society of Genetics & Plant Breeding

In situ observation of multiple levels of intermediate chromatin fibers on metaphase chromosomes of Triticum aestivum L.

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Metaphase chromosomes of root-tip meristematic cells of
wheat (Triticum aestivum L.) were carefully examined under
the transmission electron microscope. It was obvious that
only in one section of a metaphase cell, besides 10 and 25-
30 nm fibers, a serial of intermediate chromatin fibers
between 30 nm and metaphase chromosome such as 50-
60, 100-120 and 200-300 nm, could be identified on three
metaphase chromosomes in the cell.The higher degrees of
chromatin fibers were helically coiled from the lower levels
of chromatin fibers and at last, 750~800 nm metaphase
chromosomes were formed from 200-300 nm fibers. It was
concluded that the 30 nm chromatin fibers exist truly in the
metaphase chromosomes which are hierarchically
organized from the 10 nm ones and there are at least 4
levels of helical coils from 30 nm to metaphase

Keywords: Metaphase chromosome, intermediate chromatin fiber, transmission electron microscopy, Triticum aestivum L.


Year: 2015
Volume: 75
Issue: 2
Article DOI: 10.5958/0975-6906.2015.00028.0
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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