Identifying QTL for seed weight in a cross between vegetable and grain type soybeans

Published on Dec 08, 2020 03:52 AM

The variation for 100 seed weight in F2 population derived
from a cross, AGS456 (an exotic vegetable type from
Taiwan)/SL958 of soybean followed a normal curve with a
range of 8.00-27.22g indicating quantitative nature of genetic
control for seed size. Parental lines were screened with 207
SSR markers to identify polymorphism and 90 primer pairs
detected polymorphism between the parents. These ninety
markers were used for detecting polymorphism between
two extreme bulks for seed weight. Out of these, 18 primer
pairs were polymorphic for the bulks and were used for
bulk segregant analysis in 200 F2plants. One major QTL for
seed weight was identified on LG M with Sat_244 and
Satt175 as flanking markers, explaining 19.0 per cent
phenotypic variation. Two minor QTLs were also identified
on LG D1b, one in interval Satt041-Sat_069 with an estimated
phenotypic variation (R2) of 6.0 per cent and the other in
interval Sat_069-Sat_0183 estimating 7.0 per cent
phenotypic variance. The markers flanking TLs may help in
marker-assisted selection (MAS) for improvement of seed
weight in soybean after fine mapping and validation.

Keywords: Soybean, 100-seed weight, BSA, QTL, SSR markers

Info

Year: 2020
Volume: 80
Issue: 4
Article DOI: 10.31742/IJGPB.80.4.15
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

Gaurav Khosla, B. S. Gill, Asmita Sirari, Pankaj Sharma and Inderpreet Dhaliwal

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