Indian Society of Genetics & Plant Breeding

Identification of RAPD markers associated with Helminthosporium leaf blight (HLB) disease resistance in wheat

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To detect a set of RAPD markers associated with spot
blotch/ Helminthosporium leaf blight (HLB) resistance in
wheat, F2 population of a cross, WH 147 x Chirya 3,
comprising 332 individuals was analyzed by Bulked
segregant analysis (BSA). Four putative polymorphic and
reproducible RAPD markers; OPK06791, OPA101040,
OPN15765 and OPA12464 were identified. The markers,
OPK06791 and OPA101040 were associated with resistance
in repulsion phase while, OPN15765 and OPA12464 in
coupling phase. Together, these markers determined a
significant proportion of total phenotypic variation
(25.05%). Regression analysis revealed three markers,
OPK06791, OPA101040 and OPN15765 to be significantly
associated with disease phenotype.

Keywords: Helminthosporium leaf blight, spot blotch, RAPD markers, bulked segregant analysis, disease resistance


Year: 2009
Volume: 69
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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