Indian Society of Genetics & Plant Breeding

Identification of novel haplotype of a cyst nematode resistance gene, GmSNAP18 in soybean [Glycine max (L.) Merr.]

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Soybean cyst nematode (SCN) is one of the most damaging
pest of soybean. Discovery and characterization of the genes
involved in SCN resistance are important in soybean
breeding. Soluble NSF attachment protein (SNAP) genes
are related to SCN resistance in soybean. SNAP genes
include five gene families, and 2 haplotypes of exons 6 and
9 of SNAP18 are considered resistant to the SCN. In present
study the haplotypes of
GmSNAP18 were surveyed and
chacterized in a total of 60 diverse soybean genotypes
including Korean cultivars, landraces, and wild-types. The
target region of exons 6 and 9 in
GmSNAP18 region was
amplified and sequenced to examine nucleotide variation.
Characterization of 5 haplotypes identified in present study
for the
GmSNAP18 gene revealed two haplotypes as
resistant, 1 as susceptible and two as novel. A total of
twelve genotypes showed resistant haplotypes, and 45
cultivars were found susceptible. Interestingly, the two
novel haplotypes were present in 3 soybean lines. The
information provided here about the haplotypic variation
GmSNAP18 gene can be further explored for soybean
breeding to develop resistant varieties.

Keywords: Soybean cyst nematode, resistance, single nucleotide polymorphism, alignment, haplotypes


Year: 2020
Volume: 80
Issue: 3
Article DOI: 10.31742/IJGPB.80.3.5
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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