Indian Society of Genetics & Plant Breeding

Identification of markers associated with iron and zinc concentration in recombinant inbred lines of brown rice

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Association of marker and traits were studied using single
marker analysis for iron and zinc concentration in rice grains.
Grain iron and zinc concentration of rice at two locations
was estimated using Atomic Absorption Spectrometer.
Across the locations. Iron concentration ranged from 4 to
270 ppm, while that of zinc ranged from 14 to 31 ppm.
Parental polymorphism studies identified 82 (11.2%)
polymorphic markers from 735 rice microsatellites across
12 chromosomes, and 2 polymorphic markers, ZIPRM 1067
and ZIPRM 5511 out of 36 gene specific primers. The 84
markers with 126 recombinant inbred lines (F6) were used
for simple linear regression analysis. A total of 25 markers
were significantly associated with iron and/or zinc
concentration at one or both locations. Out of these,
RM3322 and RM7488 were associated with both iron and
zinc.The markers identified may be useful in future breeding
programmes aimed at biofortification in rice.

Keywords: Iron, zinc, polymorphism, recombinant inbred lines


Year: 2014
Volume: 74
Issue: 4
Article DOI: 10.5958/0975-6906.2014.00865.7
Print ISSN: 0019-5200
Online ISSN: 0975-6906


V. G. N. Tripura Venkata info_circle
K. Radhika, C. Cheralu info_circle
V. Roja and N. Sarla info_circle

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