Indian Society of Genetics & Plant Breeding

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Identification of genomic regions for salinity tolerance at germination stage of rice using doubled haploid mapping population

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Seed germination is the most sensitive stage of rice for
salinity stress. To map the genomic regions, a set of 117
doubled haploid (DH) lines derived from the F
1s of salinity
tolerant Pokkali and susceptible Savitri were used.
Preliminary screening at 0, 4, 8, 12, 16 and 20 dsm
-1of NaCl
solution, Pokkali and Savitri showed significant variation
of 78 % and 100 %, respectively for seed germination. Based
on this study, 117 DH lines were evaluated at 16 dsm
-1NaCl,
which exhibited variation in germination percentage, shoot
length and root length. Bulked segregant analysis (BSA)
was conducted by taking ten each extreme DH lines with 79
polymorphic SSR markers from 12 chromosomes to identify
the genomic regions. Three markers, RM247, RM324 and
RM283 were identified and found to be linked to four
candidate genes, LOC_Os12g06560, LOC_Os12g06570,
LOC_Os01g09550 and LOC_Os01g09560. Further research
work is suggested to understand the tolerant mechanism
of salinity at germination stage in rice

Keywords: Rice, bulked segregant analysis, doubled haploid, salinity, germination

Info

Year: 2021
Volume: 81
Issue: 2
Article DOI: 10.31742/IJGPB.81.2.14
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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