Indian Society of Genetics & Plant Breeding

Identification of fertility restorers among Assam rice cultivars by phenotyping and molecular screening approaches

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In three line hybrid rice seed production the combination
of a CMS line, maintainer line and restorer line are involved
in the development of hybrids. The wild abortive CMS is
extensively used in commercial hybrid seed production.
The fertility restoration of wild abortive CMS is governed
by two independent dominant Rf genes viz., Rf4 and Rf3.
The present study was conducted with the objective of
molecular characterization rice genotypes from Assam,
India and to identify restorers for hybrid rice breeding
programme. A total one hundred and eleven hybrids were
generated and evaluated for their pollen and spikelet fertility.
Based on molecular screening twenty five genotypes were
identified to carry only Rf4 gene and three genotypes
identified to carry both Rf3 and Rf4 genes. These identified
restorers in the present study would be a usable restores
for hybrid rice breeding in north east India. Molecular
diversity for fertility restoration using NTSYS package
formed two clusters among the tested genotypes, cluster
A represented a landrace Terabali from Assam and cluster
B with all other genotypes.

Keywords: Fertility restoration, Rf3, Rf4, WA-CMS, rice, hybrids


Year: 2016
Volume: 76
Issue: 1
Article DOI: 10.5958/0975-6906.2016.00002.X
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Pulin Borah info_circle
Revathi Ponnuswamy info_circle
Debojit Sarma info_circle
Ashutosh Roy info_circle
G. N. Hazarika info_circle

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