Indian Society of Genetics & Plant Breeding

Identification of DNA markers linked to H2 locus of fusarium wilt resistance in chickpea (Cicer arietinum L.)

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Resistance to chickpea wilt caused by Fusarium
oxysporum f.sp. ciceris race 1 is governed by two to
three genes. The DNA marker linked to H1 locus is
already available. In order to identify DNA marker linked
to H
2 locus of wilt resistance, the recombinant inbred
lines derived from the cross K 850 (late wilting) x WR-
315 (resistant), segregating for only H2 locus were
utilized. The recombinant inbred lines showed 1:1
segregation for late wilting and resistance. Seventynine random oligonucleotide primers of 10 to 11 base
pairs were used to study the polymorphism in parents.
The primer A07C amplifies an extra band of 417 bp in
susceptible parent and co-segregate in susceptible
bulk. The DNA marker A07C
417 showed monogenic
segregation ratio of 1:1 in the recombinant inbred lines.
The linkage analysis indicated that the A07C417 marker
is linked to H
2 locus and susceptibility and were
separated by 21.7 centi Morgan (eM). The RILs of
another cross JG-62 x WR-315 segregate for both H1
and H
2 loci; consequently the DNA markers linked to
H
1 and H2 also showed independent segregation in the
RILs of a cross JG-62 x WR-315. The A07C
417 marker
was also found linked to H
2 locus of wilt susceptibility
in different genotypes tested. The DNA marker A07C417
showed linkage with H2 locus across genetic
backgrounds. The identification of DNA markers linked
to both H
1 and H2 of wilt resistance will facilitate markerassisted selection and pyramiding of resistance genes
to susceptible varieties.  
Keywords: Chickpea, wilt resistance, Fusarium oxysporum, DNA marker, recombinant inbred lines

Info

Year: 2007
Volume: 67
Issue: 4
 Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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