Indian Society of Genetics & Plant Breeding

Identification of bread wheat genotypes based on high Mr weight glutenin subunits

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The seed proteins of 24 varieties/advanced breeding lines
of Indian bread wheat were fractionated using Sodium
Oodecyl Sulphate-Polyacrylamide Gel Electrophoresis
(SOS-PAGE) to determinbe their high Mr glutenin subunit
composition. Amongst the 24 varieties/advanced breeding
lines, three alleles were identified for the Glu-A 1 locus
(subunits 1,2* and null phenotype), three alleles for the
Glu-B1 locus (7+8, 7+9 and 17+18) and two for the Glu-D1
locus (2+12 and 5+10). These subunits showed high
polymorphism. Based on these subunits 9 different groups
were observed in 24 genotypes. Two genotypes viz., UP
2441 and CPAN 3004 had distinct group from the remaining
genotypes. The genotype UP 2441 showed null, 17+18
and 2+12 and CPAN 3004 revealed 1, 7+9 and 2+12
subunits. This suggested th'e usefulness of seed protein
electrophoresis showing high Mr glutenin subunits in
identifying and characterizing the different wheat

Keywords: Bread wheat, seed protein, electrophoresis, HMW subunit of glutenin


Year: 2003
Volume: 63
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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