Indian Society of Genetics & Plant Breeding

Identification of a STMS marker for sucrose content in sugarcane (Saccharum spp.)

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n order to identify molecular markers for sugar content
in sugarcane, a mapping population involving the high
sucrose parent (CoC 671) and a low sucrose parent (BC
6) was developed and phenotyped for juice quality through
evaluating in a replicated field experiment. Statistically
superior and inferior progeny for sucrose content were
identified, from which six high and low sucrose progeny
were randomly selected to constitute high and low sucrose
bulks for progeny survey. Parental polymorphism survey
with 42 sugarcane specific STMS primers generated eight
polymorphic markers (NKS 5139, NKS 9279, NKS 30379, NKS
31336, NKS 45180, 330 and NKS 46220, 345) with six primers.
Genotyping with these primers in the selected high and
low sucrose progeny led to the identification of two
markers viz., NKS45180 and NKS46220 specific to the high
sucrose bulk and in the individual progeny that constituted
the bulk. Validation of these two markers on a set of ten
high sucrose and ten low sucrose commercial clones with
different genetic backgrounds showed that the marker
NKS45180 was specific to high sucrose clones and absent
in all the low sucrose types. This marker can be used in
the selection of high sucrose clones and holds promise
in marker assisted selection and marker assisted
backcrossing to identify sucrose rich clones in the early
stages of selection.

Keywords: Sugarcane; sucrose content; mapping population; STMS marker


Year: 2012
Volume: 72
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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