Indian Society of Genetics & Plant Breeding

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Homocysteine S-methyl transferase regulates sulforaphane concentration in Brassica oleracea var. italica

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Homocysteine S-methyl transferase (HMT, EC 2.1.1.10) is a
ubiquitous enzyme in angiosperms that plays an important
role in sulfur nutrition status. We used over-expression
and RNA interference (RNAi) to study the regulatory
function of BoHMT1 on sulforaphane concentration in
broccoli. Agrobacterium-mediated transforrmation was
used to produce 63 putatively transgenic broccoli plants;
of these, 51 were positive for the gene construct by PCR
selection, and 44 were confirmed by GUS staining. Overexpression
of BoHMT1 caused a significant increase in the
relative expression level of this gene, and five independent
transgenic plants had expression levels >4-fold higher than
in the control and they also showed an increase in
sulforaphane concentration. Similarly, expression of an
RNAi construct decreased the relative expression of
BoHMT1, and four independent transgenic lines had
expression levels >4-fold lower than in the control, and
also showed a reduction in sulforaphane concentration. It
is concluded that the BoHMT1 gene regulates sulforaphane
concentration in broccoli and that there is a positive
relationship between the expression level of the gene and
the sulforaphane content.

Keywords: Brassica oleracea var. italica, homocysteine S-methyltransferase, RNA interference, sulforaphane

Info

Year: 2015
Volume: 75
Issue: 3
Article DOI: 10.5958/0975-6906.2015.00056.5
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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Juncheng Lin info_circle
Fule Xu info_circle

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