High frequency plant regeneration from an amphiploid hybrid seed of Glycine max x G. tomentella through tissue culture and hybridity testing of the regenerants

Published on Jan 16, 2021 04:09 AM

Fifty-five plants from the amphiploid hybrid (2n = 118) of
Glycine max (2n = 40) and G. tomentella (2n = 78) were
regenerated from a single seed in tissue culture. The
embryonic axis converted into a solid, green mass and
gave rise to shoot buds on 6-benzyl aminopurine (BA)-
supplemented medium. Shoots were eventually obtained
on the same medium. Rooting occurred in 100% of the
plants on Whites medium containing coumarin (9 mgr1).
A total of 50 plants survived the hardening treatment. All
of the tissue culture-raised plants were morphologically
similar and flowered under greenhouse conditions. The
hybridity of the regenerated plants was confirmed by
using morphological and simple sequence repeat (SSR)
markers. Attempts are being made to cross these plants
with soybean to generate backcross-derived lines for
further genetic studies

Keywords: Soybean, intersubgeneric hybrid, genetic stability, SSR marker

Info

Year: 2005
Volume: 65
Issue: 2
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

R. K. Tyagi, J. J. Zou and T. Hymowitz

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