Indian Society of Genetics & Plant Breeding

Hematoxylin staining of seedling roots is a potential phenotypic index for screening of aluminium tolerance in rice (Oryza sativa L.)

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Hematoxylin staining is an early indicator of Aluminium
(Al) toxicity effects on the apices of young, developing
roots and has also been used as a precocious, nondestructive way of studying Al-sensitivity in plant species.
Al(H2O)63+ which is known as Al3+ is dominant in acid soil
below pH 5.0 and is the most toxic form. Presence of Al in
the rhizosphere of rice in acid soil restricts root growth and
significantly reduces the crop productivity. In this present
study, 40 rice genotypes were screened for Al-toxicity
tolerance based on the hematoxylin staining of root apex
of rice seedlings. The genotypes were classified into
tolerant, moderately tolerant and susceptible. Based on
staining intensity the genotypes IET 22838, Gobindabhog,
Kalabhog, Khasha, Badshahbhog, Radhunipagal,
Mohanbhog, Pusa Basmati-1, UBKVR-18, UBKVR-16,
UBKVR-6 were found tolerant against Al-toxicity in the
nutrient solution at 30 ppm.

Keywords: Rice root, hematoxylin staining, aluminium tolerance


Year: 2013
Volume: 73
Issue: 2
Article DOI: 10.5958/j.0975-6906.73.2.028
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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