Conservation and genetic assessment of wild banana
relatives is important for future breeding purposes.
Haplotype network analysis was conducted to wild banana
relatives comprised Ensete glaucum, Musa acuminata and
Musa balbisianausing rbcL gene sequences. Sequences
characterization showed high conservation level (91%), low
indels (1.83%), and low parsimony informatives (3.51%).
However, it was powerfull to separate the dataset at families,
genera and species level; and moderately to separate at
intraspecies level of wild bananas. Haplotype and
nucleotide diversity of wild bananas were high. At
intraspecies level, both M. acuminata and M. balbisiana
showed high haplotype diversity but low nucelotide
diversity among haplotypes; M. acuminata has higher value
than M. balbisiana. No haplotype and nucleotide diversity
in E. glaucum. Wild bananas were separated into seven
haplotypes, with four haplogroups. Mutational pathway
revealed that E. glaucum haplotype became root; and was
closely related to M. balbisiana than M. acuminata. M.
acuminata var. malaccensis haplotype became root within
all M. acuminata varieties, and each haplotype differed by
single point mutation.
Keywords: Conservation genetic, DNA sequences, molecular, polymorphism, phylogenetic
Article DOI: 10.31742/IJGPB.80.3.9
Print ISSN: 0019-5200
Online ISSN: 0975-6906
Lia Hapsari, Dewi Ayu Lestari and Rasyadan Taufiq Probojati info_circle