Genome-wide molecular mapping and QTL analysis, validated across locations and years for charcoal rot disease incidence traits in Sorghum bicolor (L.) Moench

Published on Jul 13, 2018 12:25 PM

To assess the stability of QTLs across environments and
years and to identify the stable QTL influencing the disease
incidence, 93 RILs derived from charcoal rot resistant (E
36-1) and susceptible (SPV 86) parents of sorghum
(Sorghum bicolor L.) were used to phenotype over
locations and years to find genetic architecture of charcoal
rot disease through QTL mapping. A total of 141
polymorphic marker (48/275 SSRs, 28/410 RAPD, 65/533
gSSRs) loci were mapped onto 10 linkage groups, with an
average interlocus distance of 19 cM, and total genetic
map length of 2905 cM. Composite interval mapping (CIM)
with peak threshold of 2.0 was considered for QTL
detection. A total of nine stable quantitative trait loci (QTL)
for morphological trait along with three QTLs for
biochemical trait influencing charcoal rot disease
incidence were detected.

Keywords: EST-SSRs, charcoal rot, QTL mapping, functional markers, sorghum

Info

Year: 2012
Volume: 72
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

Ayyanagouda Patil*, Bashasab Fakrudin, Prakash M Salimath and Rajkumar

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