Indian Society of Genetics & Plant Breeding

Genetics of semi-determinacy and identification of molecular marker linked to Dt1 locus in chickpea (Cicer arietinum L.)

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Chickpea is predominantly an indeterminate (IDT) plant
due to which high fertility and irrigation have adverse effect
on seed yield. The semi-determinate (SDT) types are
relatively early, lodging resistant and found to be similar in
their yield potential to that of IDT genotypes. However, the
inheritance of SDT stem type is not well understood in
chickpea. The present investigation was aimed at studying
the genetics of SDT stem type and identifying molecular
marker linked to Dt1 locus through Bulked Segregant
Analysis (BSA). The genetics of semi-determinacy was
studied in BGD 72 (IDT) x BG 3078-1(SDT) cross in which
the genetic constitution of BGD 72 was already known as
Dt1Dt1Dt2Dt2 based on the previous study. The F1 was IDT
indicating the dominance of indeterminacy over semideterminacy. The segregation in F2 and F3 revealed that
SDT stem growth in the new-found genotype BG3078-1
was governed by a single dominant gene Dt2 and its
genotype designated as dt1dt1Dt2Dt2. The study of
polymorphic survey between BGD-72(IDT) and BG 3078-
1(SDT) using 581 SSR markers found 50 markers
polymorphic. The BSA using 50 polymorphic markers has
identified TA42 and TR29 as polymorphic between IDT and
SDT parents as well as IDT and SDT bulks and hence
considered putatively linked to Dt1 locus governing IDT
stem growth in chickpea. The two linked markers were
validated in 15 IDT and 15 SDT F2plants individually. These
two markers were also validated in 3 IDT, 8SDT and 3 DT
genotypes. This is the first report on the identification of a
molecular marker associated with stem growth habit in

Keywords: Chickpea, stem growth habit, genetics, gene tagging


Year: 2019
Volume: 79
Issue: 1, Supplement
Article DOI: 10.31742/IJGPB.79S.1.18
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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