Indian Society of Genetics & Plant Breeding

Genetics of fertility restoration and test for allelism of restorer genes in wheat (Triticum aestivum L.)

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Mode of inheritance of fertility restorer (Rf) gene(s) for T
type (Triticum timopheevi Zhuk.) sterile cytoplasm in wheat
(Triticum aestivum L.) was studied using fertility restorer
lines EC368169 and T2003R. A-line 2041A was crossed
with EC368169 while another male sterile line 2019A, with
T2003R to develop F2 and BC1 (sterile line x F1)
populations. The F1’s of all the crosses had more than 60%
pollen fertility and 48 to 68 seed set per main spike. Based
on the observations recorded on pollen and floret fertility,
the F2 and BC1 segregants were categorized into fertile (>
30% pollen fertility and > 5% seed set/spike) and sterile (<
30% pollen fertility and < 5% seed set/spike) classes. The
segregation pattern in F2 and BC1generations showed that
both the restorer lines carried a single dominant gene for
fertility restoration. However, the frequency distribution
of plants in terms of pollen fertility and seed set in the F2
indicated that fertility restoration was affected in complex
way by modifiers. Test for allelism between four restorer
lines were also conducted to find out the similarity
between the genes carried by restorer lines namely,
EC368169, T2003R, PWR4099 and PWR4101. The progenies
from three way crosses, [2019A/F1(PWR4101/PWR4099)],
[2041A/F1(PWR4101/T2003R)], [2041A/F1(WR4099/
T2003R)], 2019A/F1(EC368169R/T2003R)] and [2338A/
F1(EC368169R/PWR4099)] did not segregate for male
sterility indicating that at least one common fertility
restorer gene is present in all the four restorer lines.

Keywords: Cytoplasmic male sterility, fertility restorer genes, inheritance, test for allelism, bread wheat


Year: 2011
Volume: 71
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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